Curriculum Vitae

Personal Information

Name: Song Xue (薛嵩)
Research Focus: Novel strategies and technologies for the prevention and treatment of emerging infectious diseases
Email: 17301010038@fudan.edu.cn
ORCID: 0009-0006-5359-9928

2022.09 – Present | Fudan University | Ph.D. Candidate | Pathogen Biology Research areas:
- Antibody-dependent enhancement (ADE) evaluation of SARS-CoV-2 variants
- HBV genome heterogeneity and functional divergence in chronic hepatitis B patients
- High-throughput screening and functional validation of broadly neutralizing antibodies against SARS-CoV-2
2017.09 – 2022.06 | Fudan University | B.Sc. | Forensic Medicine Systematic training in medicine, biology, and research methodology; solid foundation in molecular biology, pathogen biology, and data analysis.

High-resolution analysis of HBV quasispecies in chronic hepatitis B patients based on targeted sequencing.
Detailed characterization of intra-host genome heterogeneity, low-frequency functional variants, and their potential biological significance.
Integration of clinical samples and bioinformatics analysis to explore the relationship between viral diversity and immune escape, antibody therapy response divergence.
Manuscript submitted to GUT (IF 26.2) — currently under review.

Investigated antibody-dependent enhancement (ADE) differences across SARS-CoV-2 variants.
Integrated self-generated Bulk RNA-seq data with scRNA-seq data from GEO/GSA databases to systematically analyze host response differences induced by different viral strains.
Characterized the impact of different variants on PBMC proportion changes, cell phenotype remodeling, and immune feature alterations in the ADE context.
Established a research framework combining in vitro functional assays + multi-omics data integration.

Conducted bioinformatics analysis based on public sequence data of common adenoviruses and enteroviruses (e.g., EV71, Coxsackie virus).
Performed alignment, screening, and evaluation of conserved regions across different pathogens to design primer/probe sequences for detection.
Supported third-party companies in developing virus colloidal gold detection kits based on ERA technology.
Possess complete practical experience from sequence collection, conserved region screening, candidate primer design to translational application support.

Long-term engagement in isolation, characterization, and functional profiling of fully human monoclonal antibodies.
Proficient in PBMC separation, flow cytometric sorting of antigen-specific single B cells, nested PCR amplification of antibody sequences, antibody expression and purification.
Capable of independently conducting antibody binding activity assays, neutralization assays, ADE-related detection, and in vivo functional evaluation.

Participated in miRNA biomarker screening for electrocution-related death research at Fudan University’s forensic medicine department.
Possess interdisciplinary research experience, able to apply experimental techniques and data analysis methods to diverse biomedical questions.

Xue, S., Han, Y., Wu, F., Wang, Q. Mutations in the SARS-CoV-2 spike receptor binding domain and their delicate balance between ACE2 affinity and antibody evasion. Protein & Cell, 2024.
Wu, J., Chen, Z., Gao, Y., Wang, Z., Wang, J., et al. Fortuitous somatic mutations during antibody evolution endow broad neutralization against SARS-CoV-2 Omicron variants. Cell Reports, 2023.
Xia, A., Wang, X., He, J., Wu, W., Jiang, W., Xue, S., et al. Cross-reactive antibody response to Monkeypox virus surface proteins in a small proportion of individuals with and without Chinese smallpox vaccination history. BMC Biology, 2023.
Jiang, W., Wu, J., He, J., Xia, A., Wu, W., Gao, Y., Zhang, Q., et al. Re-evaluation and retrospective comparison of serum neutralization induced by three different types of inactivated SARS-CoV-2 vaccines. Vaccines, 2024.

High-Resolution Profiling of HBV Quasispecies Reveals Functionally Active Low-Frequency HBV Variants Driving Immune Escape and Antibody Therapy Response. Target journal: GUT (under review)

2026.04 | Fudan University Outstanding Doctoral Student Academic Scholarship
2025.10 | Fudan University Outstanding Communist Youth League Member
2021.09 | Fudan University Zhuo-Bo Program Scholarship

Molecular Biology: Nucleic acid extraction, PCR/qPCR, vector construction, cloning, sequencing validation
Protein & Antibody: Protein expression and purification, antibody preparation, ELISA, Western blot
Virology & Immunology: Neutralization assays, ADE functional detection, antibody binding activity analysis
Animal Experiments: Animal model construction, in vivo therapeutic experiments, sample collection and efficacy evaluation
Cell Biology: PBMC isolation, flow cytometry, single B cell sorting, cell culture

High-throughput Sequencing: Bulk RNA-seq, scRNA-seq, targeted sequencing data processing and analysis
Single-cell Analysis: Cell clustering, dimensionality reduction visualization, marker identification, cell proportion comparison, cell state and phenotype analysis
Transcriptomics: Differential expression analysis, functional enrichment analysis, pathway analysis, immune-related feature comparison
Viral Sequence Analysis: Genome alignment, variant identification, conserved region screening, primer/probe design
Statistical Analysis: Common statistical tests, result visualization, data cleaning, statistical analysis, and figure output
Programming: Proficient in R and Python for data analysis, statistical modeling, and scientific visualization

Skilled in scientific figure preparation, manuscript figure assembly, and result visualization
Strong English literature reading, academic writing, and presentation abilities
Hands-on translational experience from basic research to detection application support

Focused on pathogen biology and antibody-related research, with solid experimental + bioinformatics capabilities.
Able to complete the full workflow from experimental design, sample processing, functional validation to data analysis and manuscript writing.
Strong interest in the interdisciplinary research of virology, immunology, and transcriptomics, especially in integrating experimental research with multi-omics data analysis.
Good research execution, learning ability, and teamwork awareness, with a solid foundation for paper output and sustained research potential.

Last updated on February 19, 2025